Preparation of Osazone
To confirm the presence of carbohydrate during the analysis of an organic compound, the best way would be the preparation of osazone from the original organic compound given to you.
Chemicals and Equipment’s
- 0.5 grams of carbohydrates or sugar
- 0.4 grams of phenyl hydrazibe hydrochloride
- 0.6 grams of of crystalline sodium acetate
- 50 mL pyrex test tube or a suitable test tube perferably pyrex or borosil.
- 400 mL beaker to prepare a water bath
- A water heater electric or bunsen burner
- Wire gauze if using the bunsen burner
- A tripod stand if using the bunsen burner.
Procedure
In order to prepare the compound, first you need to set up the water bath using a 400 mL beaker that is filled with about 300 mL water.
Now dissolve 0.4 grams of phenyl hydrazine and 0.6 grams of crystalline sodium acetate in 4 or 5 mL of distilled water.
Add this mixture to 0.5 grams of sugar dissolved in 3 or 4 mL of distilled water. Loosely close the test tube with a stopper to hold the reagents. Now place the test tube in the hot water bath, with gentle stirring or agitation. The time taken for the osazone to appear is a sure sign of the type of sugar used. Once the precipitation of the osazone is complete, filter the precipitate and recrystallize the osazone from 50% alcohol